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anti cd8 apc cy7 mabs  (R&D Systems)


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    R&D Systems anti cd8 apc cy7 mabs
    Figure 3 Detection of T cell subpopulations in the peripheral blood and CTL-mediated lytic activity. A, There was no difference in the percentage of CD3+ T and CD3+CD4+ T cells between the transplant groups. The number of <t>CD3+CD8+</t> T cells in the acute rejection group increased significantly (P<0.05), while the number of CD4+/CD8+ cells decreased significantly compared to the other groups (P<0.05). B, The ratio of Foxp3+CD4+CD25+ Tregs and the expression of CD4+CD25+ T cells in the acute rejection group was significantly lower than those in the other groups (P<0.05). C, In the control group, GITR expression was not detectable on T cells. GITR expression on increased T cells from all groups after culture with phorbol ester and ionomycin. The GITR expression level of T cells in the acute rejection group was significantly higher than that in the other groups (P<0.05). D, The spleen CTL-mediated lytic activity of the recipients in the acute rejection group increased by 32.6%, and was significantly higher than those in the other three groups (P<0.05).
    Anti Cd8 Apc Cy7 Mabs, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 128 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Total body irradiation of donors can alter the course of tolerance and induce acute rejection in a spontaneous tolerance rat liver transplantation model."

    Article Title: Total body irradiation of donors can alter the course of tolerance and induce acute rejection in a spontaneous tolerance rat liver transplantation model.

    Journal: Science China. Life sciences

    doi: 10.1007/s11427-012-4370-3

    Figure 3 Detection of T cell subpopulations in the peripheral blood and CTL-mediated lytic activity. A, There was no difference in the percentage of CD3+ T and CD3+CD4+ T cells between the transplant groups. The number of CD3+CD8+ T cells in the acute rejection group increased significantly (P<0.05), while the number of CD4+/CD8+ cells decreased significantly compared to the other groups (P<0.05). B, The ratio of Foxp3+CD4+CD25+ Tregs and the expression of CD4+CD25+ T cells in the acute rejection group was significantly lower than those in the other groups (P<0.05). C, In the control group, GITR expression was not detectable on T cells. GITR expression on increased T cells from all groups after culture with phorbol ester and ionomycin. The GITR expression level of T cells in the acute rejection group was significantly higher than that in the other groups (P<0.05). D, The spleen CTL-mediated lytic activity of the recipients in the acute rejection group increased by 32.6%, and was significantly higher than those in the other three groups (P<0.05).
    Figure Legend Snippet: Figure 3 Detection of T cell subpopulations in the peripheral blood and CTL-mediated lytic activity. A, There was no difference in the percentage of CD3+ T and CD3+CD4+ T cells between the transplant groups. The number of CD3+CD8+ T cells in the acute rejection group increased significantly (P<0.05), while the number of CD4+/CD8+ cells decreased significantly compared to the other groups (P<0.05). B, The ratio of Foxp3+CD4+CD25+ Tregs and the expression of CD4+CD25+ T cells in the acute rejection group was significantly lower than those in the other groups (P<0.05). C, In the control group, GITR expression was not detectable on T cells. GITR expression on increased T cells from all groups after culture with phorbol ester and ionomycin. The GITR expression level of T cells in the acute rejection group was significantly higher than that in the other groups (P<0.05). D, The spleen CTL-mediated lytic activity of the recipients in the acute rejection group increased by 32.6%, and was significantly higher than those in the other three groups (P<0.05).

    Techniques Used: Activity Assay, Expressing, Control



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    Figure 3 Detection of T cell subpopulations in the peripheral blood and CTL-mediated lytic activity. A, There was no difference in the percentage of CD3+ T and CD3+CD4+ T cells between the transplant groups. The number of <t>CD3+CD8+</t> T cells in the acute rejection group increased significantly (P<0.05), while the number of CD4+/CD8+ cells decreased significantly compared to the other groups (P<0.05). B, The ratio of Foxp3+CD4+CD25+ Tregs and the expression of CD4+CD25+ T cells in the acute rejection group was significantly lower than those in the other groups (P<0.05). C, In the control group, GITR expression was not detectable on T cells. GITR expression on increased T cells from all groups after culture with phorbol ester and ionomycin. The GITR expression level of T cells in the acute rejection group was significantly higher than that in the other groups (P<0.05). D, The spleen CTL-mediated lytic activity of the recipients in the acute rejection group increased by 32.6%, and was significantly higher than those in the other three groups (P<0.05).
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    Image Search Results


    Figure 3 Detection of T cell subpopulations in the peripheral blood and CTL-mediated lytic activity. A, There was no difference in the percentage of CD3+ T and CD3+CD4+ T cells between the transplant groups. The number of CD3+CD8+ T cells in the acute rejection group increased significantly (P<0.05), while the number of CD4+/CD8+ cells decreased significantly compared to the other groups (P<0.05). B, The ratio of Foxp3+CD4+CD25+ Tregs and the expression of CD4+CD25+ T cells in the acute rejection group was significantly lower than those in the other groups (P<0.05). C, In the control group, GITR expression was not detectable on T cells. GITR expression on increased T cells from all groups after culture with phorbol ester and ionomycin. The GITR expression level of T cells in the acute rejection group was significantly higher than that in the other groups (P<0.05). D, The spleen CTL-mediated lytic activity of the recipients in the acute rejection group increased by 32.6%, and was significantly higher than those in the other three groups (P<0.05).

    Journal: Science China. Life sciences

    Article Title: Total body irradiation of donors can alter the course of tolerance and induce acute rejection in a spontaneous tolerance rat liver transplantation model.

    doi: 10.1007/s11427-012-4370-3

    Figure Lengend Snippet: Figure 3 Detection of T cell subpopulations in the peripheral blood and CTL-mediated lytic activity. A, There was no difference in the percentage of CD3+ T and CD3+CD4+ T cells between the transplant groups. The number of CD3+CD8+ T cells in the acute rejection group increased significantly (P<0.05), while the number of CD4+/CD8+ cells decreased significantly compared to the other groups (P<0.05). B, The ratio of Foxp3+CD4+CD25+ Tregs and the expression of CD4+CD25+ T cells in the acute rejection group was significantly lower than those in the other groups (P<0.05). C, In the control group, GITR expression was not detectable on T cells. GITR expression on increased T cells from all groups after culture with phorbol ester and ionomycin. The GITR expression level of T cells in the acute rejection group was significantly higher than that in the other groups (P<0.05). D, The spleen CTL-mediated lytic activity of the recipients in the acute rejection group increased by 32.6%, and was significantly higher than those in the other three groups (P<0.05).

    Article Snippet: A 50 μL sample of the whole blood obtained after stimulation was incubated with 10 μL of anti-CD3-PerCp and 5 μL of anti-CD8-APC-Cy7 mAbs in the dark at 4°C for 30 min. After haematolysis and washing with PBS, the resuspended cells were incubated in the dark at 4°C for 45 min with 10 μL anti-rat GITR-PE mAb (R&D Systems, Minneapolis, MN, USA) or 10 μL IgG1-PE (Beckman Coulter Inc) as an isotype-control.

    Techniques: Activity Assay, Expressing, Control